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Part:BBa_K187090

Designed by: Julia Pon   Group: iGEM09_Alberta   (2009-10-21)

terminator forward strand B, Biobytes assembly method

This part can be annealed to the terminator reverse strand (BBa_K187077), to produce a B overhang, which can anneal to the B' end on the 3' end of a part produced from pAB.

The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct.

For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or RFC #47

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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